Journal: Emerging Infectious Diseases

Article Title: Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus

doi: 10.3201/eid2302.161239

Figure Lengend Snippet: Presence of MERS-CoV receptor DPP4 (IHC) and of mucosubstances (PAS) in upper and lower respiratory tract tissues from sheep, pigs, llamas, and horses. A) In the nose, DPP4 (red cytoplasmic or membrane staining) was present on the lining epithelium of pigs, llamas, and horses but not sheep. PAS staining (magenta) demonstrated more mucous cells in the lining epithelium of sheep and horses and a layer of mucus on the lining epithelium of the horses. B) DPP4 (red cytoplasmic or membrane staining) was present on the lining epithelium of the trachea, bronchus/bronchioles, and alveoli in the pigs, llamas and horses but not in the sheep. Original magnification ×400 for all images. DPP4, dipeptidyl peptidase-4; IHC, immunohistochemistry; MERS-CoV, Middle East respiratory syndrome coronavirus; PAS, periodic acid–Schiff; term., terminal.

Article Snippet: In brief, we performed DPP4 IHC staining by using 5 μg/mL of polyclonal goat IgG anti-human DPP4 antibody (R&D Systems, Abingdon, UK) and peroxidase-labeled rabbit anti-goat IgG (1:200; DAKO; Agilent Technologies Company, Santa Clara, CA, USA) as a secondary antibody.

Techniques: Membrane, Staining, Immunohistochemistry

Journal: Emerging Infectious Diseases

Article Title: Domestic Pig Unlikely Reservoir for MERS-CoV

doi: 10.3201/eid2306.170096

Figure Lengend Snippet: Comparison of the amino acid residues shown to be essential in binding of Middle East respiratory syndrome coronavirus spike protein to DPP4 of human, dromedary camel, and domestic pig*

Article Snippet: We investigated whether DPP4 is expressed in the pig respiratory tract by performing immunohistochemical staining on the nasal mucosa and lung tissue obtained from healthy pigs using an antibody against DPP4 (mouse monoclonal anti-DPP4 [CD26], clone OTI11D7, 1:2,500; Origene Technologies, Inc., Rockville, MD, USA).

Techniques: Comparison, Binding Assay

Journal: Emerging Infectious Diseases

Article Title: Domestic Pig Unlikely Reservoir for MERS-CoV

doi: 10.3201/eid2306.170096

Figure Lengend Snippet: Dipeptidyl peptidase (DPP) 4 expression in the domestic pig respiratory tract. Tissues were stained by using a cross-reactive mouse monoclonal antibody against DPP4 (CD26, clone OTI11D7, 1:2,500; Origene Technologies, Inc., Rockville, MD, USA). DPP4 expression was absent in the nasal mucosa (A) but present in lung tissue (B) of healthy domestic pigs. Original magnification: nasal mucosa ×40; lung ×200.

Article Snippet: We investigated whether DPP4 is expressed in the pig respiratory tract by performing immunohistochemical staining on the nasal mucosa and lung tissue obtained from healthy pigs using an antibody against DPP4 (mouse monoclonal anti-DPP4 [CD26], clone OTI11D7, 1:2,500; Origene Technologies, Inc., Rockville, MD, USA).

Techniques: Expressing, Staining

Journal: Hypertension

Article Title: Dipeptidyl Peptidase IV Regulates Proliferation of Preglomerular Vascular Smooth Muscle and Mesangial Cells

doi: 10.1161/hypertensionaha.112.196501

Figure Lengend Snippet: Figure 1. A, Dipeptidyl peptidase IV (DPPIV) mRNA levels in spontaneously hypertensive rat (SHR) and Wistar-Kyoto rat (WKY) preglomerular microvascular smooth muscle cells (PGVSMCs) and glomerular mesangial cells (GMCs). DPPIV mRNA expression was determined by quantitative real-time PCR and normalized to -actin mRNA. B, Western blotting for DPPIV protein using 2 different antibodies (Ab 1 and 2). A dis- tinct and single band was observed at 55 kDa for both WKY and SHR PGVSMCs and GMCs. C, DPPIV protein expression in SHR and WKY PGVSMCs and GMCs. DPPIV protein expression was quantified by densitometry analysis of Western blots and normalized to -actin protein. Letter above bars (a) indicates significantly different (Fisher least significant difference [LSD] test) from PGVSMCs.

Article Snippet: To ensure that the signal detected by Western blotting was indeed DPPIV, we performed Western blotting for DPPIV protein as described in Jackson et al.3 using two different primary antibodies: 1) rabbit polyclonal anti-DPPIV antibody (Proteintech Group, Inc.; Chicago, IL; catalog No. 10940-1-AP; 1:600); and 2) rabbit polyclonal anti-DPPIV antibody (Thermo Scientific, Rockford, IL; catalogue number PA1-8455; 1:2500).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Oncotarget

Article Title: Synergistic cytotoxic activity of cannabinoids from cannabis sativa against cutaneous T-cell lymphoma (CTCL) in-vitro and ex-vivo

doi: 10.18632/oncotarget.27528

Figure Lengend Snippet: PBL were isolated from blood samples of Sézary patients ( n = 6), and were treated with S4 (5 μg/mL), S5 (6 μg/mL), S4 (5 μg/mL) +S5 (6 μg/mL) and control (methanol; Vehicle treatment) for 48 h. Cells were harvested and analyzed by FACS following CD4-APC, CD26-alexa 405, Annexin V-FITC and PI staining. Apoptotic-induced cells (Annexin positive cells) were determined in the CD4 + CD26 - cell population and in non-CD4 + CD26 - cells of treated cells minus control. ( A ) The percent of apoptotic-induced CD4 + CD26 - cells is presented for single treatment compared to combined treatment; ( n = 4); *** denote significant difference between means (one way ANOVA; p < 0.001). ( B ) The percent of apoptotic-induced cells following the combined treatment was compared between CD4 + CD26 - cells and non-CD4 + CD26 - cells of SPBL; ( n = 6); ** denote significant difference between means (paired student T test; 0.001 < P < 0.05).

Article Snippet: Cells were suspended in 100 μL binding buffer with 1 μL Annexin V-FITC (4830-01-1, eBioscience) + 2 μL CD26 Alexa Fluor 405-conjugated antibody (FAB1180V-100UG, R&D SYSTEMS) + 10 μL CD4-APC-conjugated antibody (FAB3791A-100, R&D SYSTEMS) and incubated for 15 min at room temperature.

Techniques: Isolation, Control, Staining